AbGenics Anti-infective Camelid Antibody Pipelines

• Sixteen immunized Camelid libraries(10⁷diversity) against pathogens ( clinical isolates) from which CaMabs against surface and intercellular antigens have been isolated and used for various applications . More antibodies can be isolated on demand within a few weeks.
• Naive camelid library of 10⁹ diversity was generated from the pooled lymphocytes of 5 camels.
• Naive camelid hyperphage library of 10⁸ diversity (from 5 camels) has higher binding affnity and has a higher hit rate than the regular library. Antibodies against non-immunogenic antigens has been isolated from these libraries.
• Immunised camelid libraries against top five cancers and autoimmune tissues of Indian origin. Hits isolated against clinically important targets. 

ScFv fragment antibody libraries

• Naive Human scfv library of 10⁸ diversity generated from the B lymphocytes of 20 healthy donors. This can be used to isolate human scfvs against antigens of interest and reformatted to fully human antibodies for therapeutic applications.
• CaMabs against anti-inflammatory targets (TNF-alpha and IL6) isolated from the Naive Camel hyperphage library.
  
• Immunized mouse scfv library against cancer tissue of Indian origin. scfvs binding specifically to the cancer tissues have been isolated by phage display. These are being reformatted into full length chimeric antibodies.

Unique Engineered Molecules ( AbTids )

• Anti Candida CaMabs fused with cleavable linker to antifungal peptides.
• Anti Pseudomonas CaMabs  fused with cleavable linker to antibacterial peptides .
• Anti Staphylococcus CaMabs fused with cleavable linker to antibacterial peptides
• Anti Propyinobacter CaMabs fused with cleavable antibacterial peptides.

These AbTids are prodrugs that home to the pathogens in question and release the peptides  on contact with the pathogens, neutralising it.  

AbTids are being generated against all the ESKAPE, fungal and enteral pathogens. 

Delivery Technologies

Probiotics based delivery technology to the gut:

1. Lactobacilli and other probiotics secrete the therapeutic molecules in the gut that are absorbed by the host. Vectors have been developed to secrete or surface display therapeutic molecules in the gut. A lactobacillus displaying anti-Salmonella CaMab has been tested in vivo.
2. Lactobacillus RNAi vectors that produce target specific RNAi molecues in the gut. A Lactobacillus secreting anti-Ancylostoma RNAi was successfully tested in vivo.

These vector systems can be used to deliver other therapeutic molecules and RNAi molecules against other gut pathogens and parasites. 

Intellectual property/Publications

PATENT 1:

• Patent application no 1100/MUM/2014, 27 March , 2014 Expression of a single chain antibody against Salmonella in Lactobacillus.
• Inventors: Dr Sambuddha Ghosh, Dr Sanjiban K. Banerjee and Manisha Sabnis
• PCT, US and European Patent Filed.

PATENT 2:

• Patent Application No.116/MUM/2015 dated 13-01-2015. An Antimicrobial Combination of Antibodies and antimicrobial Peptides to Target Microbes. Full patent filed on 13-01-2016.
  Granted on 25/03/22 No: 3933037 
• Inventor: Sanjiban K Banerjee
• IP assigned to: AbGenics Life Sciences Pvt Ltd
  

PATENT 3:

• Patent Application No.  201621034928 dated 12/10/2016. A method of controlling helminth infection by probiotic mediated RNA interference. 
  
• Inventor: Sanjiban K Banerjee 
• IP assigned to: AbGenics Life Sciences Pvt Ltd

PATENT 4

• Patent Application No: 202021020768. PCT No : PCT / IB2021/05/29/89 . An antibody fragment based antifungal conjugate selectively targeting Candida. Full patent filed on 12/05/ 2020. Granted Sept 2022. No: 406119 US patent filed Nov 2022 . Application No : 17/998,347
• Inventor: Sanjiban K Banerjee
• IP assigned to: AbGenics Life Sciences Pvt Ltd
  

PATENT 5:

• Patent Application No: 202021020769. An antibody fragment based antifungal conjugate selectively targeting Pseudomonas. Full patent filed on 12/05/2020. Granted in August 2022, No 404077 US patent filed Nov 2022 . Application No : 17/998,345
•  Inventor: Sanjiban K Banerjee.
• IP assigned to: AbGenics Life Sciences Pvt Ltd

Papers under review  

1. RNA Interference Mediated Hookworm Control by Gut Dwelling Lactic Acid Bacteria

Shaziya Bi,  Manisha Sabnis, and Anamika Singh, Sanjiban K Banerjee

2. Reversal of Carbapenem Resistance in Pseudomonas aeruginosa by Single Domain Antibody Fragment (VHH)  

Anil Kumar Nagraj, Manjiri Shukla, Mansi Kulkarni, Sanjiban K Banerjee