Human short chain variable fragments are generated by combining the light and the heavy chains from the antibody repertoire of patients afflicted by various diseases. Additional diversity is generated by this recombination event that has the potential of generating antibodies with unique properties. 

Since these molecules are derived from true human libraries, the immuno toxicity issues associated with the antibodies are minimized. After selection of the desired antibodies by phage display, the scFvs can be reformatted into full length antibodies that ensure immune engagement and can be used for therapeutic applications